2024-2025 Seminars
Up Next:
Monday, October 7 | 4 p.m. — Joe Yesselman, Ph.D. | University of Nebraska-Lincoln
Monday, October 7 | 4 p.m.
In-Person: Kahn Auditorium, BSRB
Hybrid: Click here to join via Zoom.
Talk Title:
“High-throughput determination of RNA tertiary contact thermodynamics by quantitative DMS chemical mapping”
Abstract:
Structured RNAs often contain long-range tertiary contacts that are critical to their function. Despite the importance of tertiary contacts, methods to measure their thermodynamics are low throughput or require specialized instruments. Here, we introduce a new quantitative chemical mapping method (qMaPseq) to measure Mg2+-induced formation of tertiary contact thermodynamics in a high-throughput manner using standard biochemistry equipment. With qMaPseq, we measured the ΔG of 98 unique tetraloop/tetraloop receptor (TL/TLR) variants in a one-pot reaction. These results agree well with measurements from specialized instruments (R2=0.64). Furthermore, the DMS reactivity of the TL directly correlates to the stability of the contact (R2=0.68), the first direct evidence that a single DMS reactivity measurement reports on thermodynamics. Combined with structure prediction, DMS reactivity allowed the development of experimentally accurate 3D models of TLR mutants. These results demonstrate that qMaPseq is broadly accessible, high-throughput, and directly links DMS reactivity to thermodynamics.
2024
Monday, September 23 | 4 p.m. — Gregor Neuert, Ph.D. | Vanderbilt University
Monday, October 7 | 4 p.m. — Joe Yesselman, Ph.D. | University of Nebraska-Lincoln
Monday, October 21 — Laura Scott, Ph.D. | University of Michigan
Monday, November 4 — Nils Walter, Ph.D. | University of Michigan
Monday, November 18 — Jennifer Phillips-Cremins, Ph.D. | University of Pennsylvania
Monday, December 2 | 4 p.m. — Yan Zhang, Ph.D. | University of Michigan
2025
Monday, February 17, 2025 | 4 p.m. — Sundeep Kalantry, Ph.D. | University of Michigan
Monday, April 28, 2025 | 4 p.m. — Connie Wu, Ph.D. | University of Michigan