The University of Michigan Bru-seq Lab is a core facility that provides nascent RNA sequencing using four techniques: Bru-seq, BruChase-seq, BruUV-seq, and BruDRB-seq. This service is all-inclusive, starting with cells and ending with basic data analysis. In addition, users will have access to our exclusive pipeline to perform further data analysis.
Our areas of expertise are RNA isolation, cDNA library preparation, and sequencing data analysis.
We serve researchers from the University of Michigan, and other institutions in the United States and around the world.
Visit our iLabs page (with your U-M login) and schedule your service here: https://umich.corefacilities.org/service_center/show_external/5763
Services Offered
Sequencing techniques:
Bru-seq (RNA transcription)
BruChase-seq (RNA Stability)
BruUV-seq (identification of enhancers and transcription start sites)
BruDRB-seq (RNA elongation rates)
Services include:
Bromouridine labeling of cells and cell collection
Total and nascent RNA isolation
cDNA Library Preparation
Sequencing (using the University of Michigan DNA Sequencing Core)
Sample mapping and data analysis
Rates:
U-M internal: $300 per sample
External Academic $500 per sample
External Private/Comercial $500 per sample
Contact
For project initiation: Mats Ljungman
For data analysis information: Karan Bedi
For all other information (sample dropoff, technical questions, etc): Michelle Paulsen
Ordering through iLabs: https://umich.corefacilities.org/service_center/show_external/5763
Website: https://sites.google.com/a/umich.edu/ljungman-lab/
Phone: 734-232-5438 OR 734-764-3330
Email: BruseqCenter@umich.edu
Locations: 1361 and 1410 B520 NCRC
Photo: Stained gel used to analyze the quality of Bru-seq
