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Center for RNA Biomedicine

Ann Arbor, MI

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You are here: Home / Research / Core Facilities / Bru-Seq Lab

Bru-Seq Lab

The University of Michigan Bru-seq Lab is a core facility that provides nascent RNA sequencing using four techniques: Bru-seq, BruChase-seq, BruUV-seq, and BruDRB-seq. This service is all-inclusive, starting with cells and ending with basic data analysis. In addition, users will have access to our exclusive pipeline to perform further data analysis.

Our areas of expertise are RNA isolation, cDNA library preparation, and sequencing data analysis.
We serve researchers from the University of Michigan, and other institutions in the United States and around the world.

Services Offered

Sequencing techniques:
Bru-seq (RNA transcription)
BruChase-seq (RNA Stability)
BruUV-seq (identification of enhancers and transcription start sites)
BruDRB-seq (RNA elongation rates)

Services include:
Bromouridine labeling of cells and cell collection
Total and nascent RNA isolation
cDNA Library Preparation
Sequencing (using the University of Michigan DNA Sequencing Core)
Sample mapping and data analysis

Contact

For project initiation: Mats Ljungman
For data analysis information: Karan Bedi
For all other information (sample dropoff, technical questions, etc): Michelle Paulsen

Website: https://sites.google.com/a/umich.edu/ljungman-lab/
Phone: 734-232-5438  OR 734-764-3330
Email: BruseqCenter@umich.edu
Locations: 1361 and 1410 B520 NCRC

 

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