BEGIN:VCALENDAR VERSION:2.0 PRODID:-//Center for RNA Biomedicine - ECPv6.15.17//NONSGML v1.0//EN CALSCALE:GREGORIAN METHOD:PUBLISH X-WR-CALNAME:Center for RNA Biomedicine X-ORIGINAL-URL:https://rna.umich.edu X-WR-CALDESC:Events for Center for RNA Biomedicine REFRESH-INTERVAL;VALUE=DURATION:PT1H X-Robots-Tag:noindex X-PUBLISHED-TTL:PT1H BEGIN:VTIMEZONE TZID:America/Detroit BEGIN:DAYLIGHT TZOFFSETFROM:-0500 TZOFFSETTO:-0400 TZNAME:EDT DTSTART:20200308T070000 END:DAYLIGHT BEGIN:STANDARD TZOFFSETFROM:-0400 TZOFFSETTO:-0500 TZNAME:EST DTSTART:20201101T060000 END:STANDARD BEGIN:DAYLIGHT TZOFFSETFROM:-0500 TZOFFSETTO:-0400 TZNAME:EDT DTSTART:20210314T070000 END:DAYLIGHT BEGIN:STANDARD TZOFFSETFROM:-0400 TZOFFSETTO:-0500 TZNAME:EST DTSTART:20211107T060000 END:STANDARD BEGIN:DAYLIGHT TZOFFSETFROM:-0500 TZOFFSETTO:-0400 TZNAME:EDT DTSTART:20220313T070000 END:DAYLIGHT BEGIN:STANDARD TZOFFSETFROM:-0400 TZOFFSETTO:-0500 TZNAME:EST DTSTART:20221106T060000 END:STANDARD BEGIN:DAYLIGHT TZOFFSETFROM:-0500 TZOFFSETTO:-0400 TZNAME:EDT DTSTART:20230312T070000 END:DAYLIGHT BEGIN:STANDARD TZOFFSETFROM:-0400 TZOFFSETTO:-0500 TZNAME:EST DTSTART:20231105T060000 END:STANDARD END:VTIMEZONE BEGIN:VEVENT DTSTART;TZID=America/Detroit:20210921T120000 DTEND;TZID=America/Detroit:20210921T130000 DTSTAMP:20260403T160040 CREATED:20210901T190819Z LAST-MODIFIED:20210908T165943Z UID:9313-1632225600-1632229200@rna.umich.edu SUMMARY:BiolChem Seminar: Alexis Komor\, UCSD DESCRIPTION:“Base Editing-Performing Chemistry on the Genome”\nAlexis Komor\, UCSD\nDepartment of Biological Chemistry seminar\nLocation: Zoom Webinar https://umich.zoom.us/j/91821866007\nHosts: Yan Zhang and Nils Walter URL:https://rna.umich.edu/events/alexis-komor/ END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20210922T160000 DTEND;TZID=America/Detroit:20210922T170000 DTSTAMP:20260403T160040 CREATED:20210901T191748Z LAST-MODIFIED:20210901T191748Z UID:9322-1632326400-1632330000@rna.umich.edu SUMMARY:RNA Collaborative Seminar Series // Host: NCI RNA Biology Initiative DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-nci-rna-biology-initiative/ END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20210924T100000 DTEND;TZID=America/Detroit:20210924T110000 DTSTAMP:20260403T160040 CREATED:20210901T193132Z LAST-MODIFIED:20210901T193132Z UID:9344-1632477600-1632481200@rna.umich.edu SUMMARY:Jayakrishnan Nandakumar\, MCDB DESCRIPTION:“Structure of a meiosis-specific complex central to BRCA2 localization at recombination sites”\nJayakrishnan Nandakumar\, Ph.D.\nU-M LSI Structure Series\nLocation: LSI Library URL:https://rna.umich.edu/events/jk-nandakumar/ END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20211004T160000 DTEND;TZID=America/Detroit:20211004T170000 DTSTAMP:20260403T160040 CREATED:20210827T134403Z LAST-MODIFIED:20210930T134032Z UID:9267-1633363200-1633366800@rna.umich.edu SUMMARY:RNA Innovation Seminar: Byron Purse\, Ph.D. DESCRIPTION:“Fluorescent nucleoside analogues with new properties”\nByron Purse\, Ph.D.\nAssociate Professor\nOrganic Chemistry\nSan Diego State University \n  \n  \n  \n  \nHYBRID SEMINAR:\nIn-person: Forum Hall\, Palmer Commons\nZoom: https://umich.zoom.us/webinar/register/WN__vvE2dtHQi-R3h05JUHBzQ \nFLYER IN PDF \nAbstract:\nFluorescent nucleoside analogues (FNAs) are powerful probes for studying the structure and dynamics of nucleic acids\, which are vital to understanding RNA function\, DNA damage repair\, nucleic acid–protein interactions\, regulatory mechanisms for gene expression\, and other aspects of nucleic acid function. Existing FNAs are prone to quenching by base pairing and stacking\, are clustered at the blue–green end of the visible spectrum\, and have limited brightness as compared with conventional fluorophores. Studies of nucleic acid function would benefit greatly from overcoming these limitations. We have designed\, synthesized\, and studied a series of fluorescent pyrimidine analogues\, aiming to address these limitations and develop a detailed understanding of the relationships between chemical structure and fluorescent responses to local environment in nucleic acids. Included in this series is a tricyclic cytidine analogue DEAtC that is nearly non-fluorescent as a nucleoside\, but responds to matched base pairing and stacking with a fluorescence turn-on. A chlorinated tricyclic cytidine 8-Cl-tCO reports on local environment by changes in the vibrational fine structure of its emission spectra. To address the problem of limited brightness\, we have design and synthesized a new NFA that we call ABN\, which has a conjugated push–pull system similar to those found in bright fluorophores such as rhodamines. ABN is the brightest known FNA when present in duplex nucleic acids\, and it is readily detected in single-molecule fluorescence measurements using both 1-photon and 2-photon excitation. Collectively\, these FNAs offer new capabilities for biophysical studies on nucleic acids. Comparisons of their structure and properties help to reveal mechanisms for fluorescence changes in response to local environment in nucleic acids. URL:https://rna.umich.edu/events/byron-purse/ CATEGORIES:Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20211005T150000 DTEND;TZID=America/Detroit:20211005T160000 DTSTAMP:20260403T160040 CREATED:20210901T193912Z LAST-MODIFIED:20210929T155531Z UID:9346-1633446000-1633449600@rna.umich.edu SUMMARY:Human Genetics Seminar: Rajesh Rao\, Ophthalmology & Visual Science\, U-M Medical School DESCRIPTION:“Genetics through the Lens of an Ophthalmologist and Vision Scientist”\nRajesh C. Rao\, M.D.\nLeonard G. Miller Professor\nAssociate Professor\nDepartment of Ophthalmology & Visual Sciences\nDepartment of Pathology \n  \nDepartment of Human Genetics seminar\nZOOM MEETING ID: 947 5800 7294\nPASSCODE: 900076\nhttps://umich.zoom.us/j/94758007294?pwd=Sk9VMGlnTXRZWWJwbnZCZUx4MlI0QT09 \nFLYER IN PDF URL:https://rna.umich.edu/events/rajesh-rao/ CATEGORIES:Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20211006T090000 DTEND;TZID=America/Detroit:20211006T100000 DTSTAMP:20260403T160040 CREATED:20210901T191902Z LAST-MODIFIED:20210901T191902Z UID:9325-1633510800-1633514400@rna.umich.edu SUMMARY:RNA Collaborative Seminar Series // Host: NUS-CSI Singapore RNA Biology Center DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-nus-csi-singapore-rna-biology-center/ END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20211012T120000 DTEND;TZID=America/Detroit:20211012T130000 DTSTAMP:20260403T160040 CREATED:20210901T185022Z LAST-MODIFIED:20210908T170009Z UID:9294-1634040000-1634043600@rna.umich.edu SUMMARY:BiolChem Seminar: Sam Sternberg\, Columbia University DESCRIPTION:“Functional Interplay Between CRISPR-Cas and Transposable Elements”\nSam Sternberg\, Columbia University\nDepartment of Biological Chemistry seminar\nLocation: 3330 MS I\nHosts: Yan Zhang and Nils Walter URL:https://rna.umich.edu/events/sam-sternberg/ END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20211018T160000 DTEND;TZID=America/Detroit:20211018T170000 DTSTAMP:20260403T160040 CREATED:20210827T134441Z LAST-MODIFIED:20210930T165005Z UID:9269-1634572800-1634576400@rna.umich.edu SUMMARY:RNA Innovation Seminar: Tim Stasevich\, Ph.D. DESCRIPTION:“Imaging single-mRNA translation dynamics in living color”\nTim Stasevich\, Ph.D.\nAssociate Professor\nDean and Pingping Tsao Professor of Biochemistry\nCSU Monfort Professor\nBoettcher Investigator\nColorado State University \nRegistration: https://umich.zoom.us/webinar/register/WN_OzTuwf2zRN-6zCZk1pA_lQ \n  \nFlyer in PDF \nKeywords:\ntranslational regulation\, gene expression\, fluorescence microscopy\, intrabodies\, single-molecule imaging \nAbstract:\nMy lab is creating technology to image mRNA translation in real time and with single-molecule precision in living cells. In this talk\, I will introduce our technology and describe how it can be used to amplify fluorescence from newly synthesized proteins as they are being translated from single mRNAs. I will show how we quantify these signals to determine the size\, shape\, subcellular localization\, and mobilities of mRNA translation sites\, as well as their protein synthesis dynamics. I will then highlight a few recent applications of our technology\, focusing mainly on a new biosensor we have developed to quantify how individual regulatory factors impact single mRNA translation dynamics. Using this biosensor\, we provide evidence that human Argonaute2 (Ago2) shuts down translation by down regulating translation initiation on the minutes timescale and helping usher translationally silent mRNAs into P-bodies on the hours timescale. I will conclude by discussing new fluorescent intrabodies my lab is engineering to light up nascent and mature proteins in multiple colors. As these intrabodies can be encoded on plasmids\, they can easily be adapted by other labs to image gene activity in diverse living systems. \nTimothy J. Stasevich is an Associate Professor in the Department of Biochemistry and Molecular Biology at Colorado State University (CSU). His lab uses a combination of advanced fluorescence microscopy\, genetic engineering\, and computational modeling to study the dynamics of gene regulation in living mammalian cells. His lab helped pioneer the imaging of real-time single-mRNA translation dynamics in living cells1. Dr. Stasevich received his B.S. in Physics and Mathematics from the University of Michigan\, Dearborn\, and his Ph. D. in Physics from the University of Maryland\, College Park. He transitioned into experimental biophysics as a post-doctoral research fellow in the laboratory of Dr. James G. McNally at the National Cancer Institute. During this time\, he developed technology based on fluorescence microscopy to help establish gold-standard measurements of live-cell protein dynamics. Dr. Stasevich next moved to Osaka University\, where he worked with Dr. Hiroshi Kimura as a Japan Society for the Promotion of Science Foreign Postdoctoral Research Fellow. While there\, he helped create technology to image endogenous proteins and their post-translation modifications in vivo. This allowed him to image the live-cell dynamics of epigenetic histone modifications during gene activation for the first time2. Before joining the faculty at CSU\, Dr. Stasevich spent a year as a Visiting Fellow at the HHMI Janelia Research Campus\, where he applied superresolution fluorescence microscopy to improve the spatiotemporal resolution of endogenous protein imaging in live cells. \n1. Morisaki\, T. et al. Real-time quantification of single RNA translation dynamics in living cells. Science 352\, 1425–1429 (2016).\n2. Stasevich\, T. J. et al. Regulation of RNA polymerase II activation by histone acetylation in single living cells. Nature 516\, 272–275 (2014). URL:https://rna.umich.edu/events/tim-stasevich/ CATEGORIES:Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20211020T090000 DTEND;TZID=America/Detroit:20211020T100000 DTSTAMP:20260403T160040 CREATED:20210901T192024Z LAST-MODIFIED:20210901T192024Z UID:9327-1634720400-1634724000@rna.umich.edu SUMMARY:RNA Collaborative Seminar Series // Host: Helmholtz Institute for RNA-based Infection Research DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-helmholtz-institute-for-rna-based-infection-research/ END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20211026T120000 DTEND;TZID=America/Detroit:20211026T130000 DTSTAMP:20260403T160040 CREATED:20210901T185327Z LAST-MODIFIED:20210908T170049Z UID:9297-1635249600-1635253200@rna.umich.edu SUMMARY:BiolChem Seminar: Michael Terns\, University of Georgia DESCRIPTION:“Know Thy Enemy: Making CRISPR Memories”\nMichael Terns\, University of Georgia\nDepartment of Biological Chemistry seminar\nLocation: 3330 MS I\nHosts: Yan Zhang and Nils Walter URL:https://rna.umich.edu/events/michael-terns/ END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20211103T160000 DTEND;TZID=America/Detroit:20211103T170000 DTSTAMP:20260403T160040 CREATED:20210901T192135Z LAST-MODIFIED:20210901T192135Z UID:9329-1635955200-1635958800@rna.umich.edu SUMMARY:RNA Collaborative Seminar Series // Host: UCSC Center for Molecular Biology of RNA DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-ucsc-center-for-molecular-biology-of-rna/ END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20211108T160000 DTEND;TZID=America/Detroit:20211108T170000 DTSTAMP:20260403T160040 CREATED:20210827T134511Z LAST-MODIFIED:20211025T164624Z UID:9271-1636387200-1636390800@rna.umich.edu SUMMARY:RNA Innovation Seminar: Zhonggang Hou & Jun Hee Lee DESCRIPTION:“Harnessing diverse compact CRISPR-Cas3 for long-range genome engineering”\nZhonggang Hou\, Ph.D.\nResearch Investigator\nBiological Chemistry \nABSTRACT: Leading CRISPR-Cas technologies employ Cas9 and Cas12 enzymes that generate RNA-guided dsDNA breaks. Yet\, the most abundant microbial adaptive immune systems\, Type I CRISPR\, is under-exploited for eukaryotic application. I will discuss our effort on adopting the first minimal CRISPR-Cas3 from Neisseria Type I-C system\, to create targeted large chromosomal deletions in human cells. RNP delivery of the processive Cas3 nuclease and target recognition complex Cascade\, gave up to 90% editing efficiency. Unexpectedly\, Type I-C Cascade assembly in bacteria requires a previous unknown internal translation product Cas11 from within the cas8 gene. Our data show that expression of a separately encoded Cas11 is the key to enable plasmid- and mRNA- based editing in human cells. We demonstrate that “supplying cas11” is a universal strategy to harness divergent and streamlined Type I-C\, I-D and I-B editors with distinct PAM preferences and guide orthogonality. Our findings expand the CRISPR toolbox for long-range genome engineering. \nKEYWORDS: CRISPR; genome editing; Cascade; Cas3; Cas11; DNA targeting; crRNA; large deletion; Neisseria; genome engineering \n  \n  \n“Microscopic Examination of Spatial Transcriptome through Seq-Scope”\nJun Hee Lee\, Ph.D.\nAssociate Professor\nMolecular & Integrative Physiology \nKEYWORDS: Spatial Transcriptomics\, Seq-Scope \n  \nRegistration: https://umich.zoom.us/webinar/register/WN_qBK6mw7vQa6jOkZuS81_VQ \nFLYER IN PDF URL:https://rna.umich.edu/events/zhonggang-hou-and-jun-hee-lee/ CATEGORIES:Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20211109T120000 DTEND;TZID=America/Detroit:20211109T130000 DTSTAMP:20260403T160040 CREATED:20210901T185606Z LAST-MODIFIED:20210908T170232Z UID:9302-1636459200-1636462800@rna.umich.edu SUMMARY:BiolChem Seminar: Ailong Ke\, Cornell University DESCRIPTION:“Structural Basis of Bacterial CRISPR Immunity”\nAilong Ke\, Cornell University\nDepartment of Biological Chemistry seminar\nLocation: 3330 MS I\nHosts: Yan Zhang and Nils Walter URL:https://rna.umich.edu/events/ailong-ke/ END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20211117T160000 DTEND;TZID=America/Detroit:20211117T170000 DTSTAMP:20260403T160040 CREATED:20210901T192222Z LAST-MODIFIED:20210901T192222Z UID:9331-1637164800-1637168400@rna.umich.edu SUMMARY:RNA Collaborative Seminar Series // Host: The RNA Institute\, University At Albany DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-the-rna-institute-university-at-albany/ END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20211201T160000 DTEND;TZID=America/Detroit:20211201T170000 DTSTAMP:20260403T160040 CREATED:20210901T192308Z LAST-MODIFIED:20210901T192308Z UID:9333-1638374400-1638378000@rna.umich.edu SUMMARY:RNA Collaborative Seminar Series // Host: Penn RNA Group - University of Pennsylvania DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-penn-rna-group-university-of-pennsylvania/ END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20211207T120000 DTEND;TZID=America/Detroit:20211207T130000 DTSTAMP:20260403T160040 CREATED:20210901T190413Z LAST-MODIFIED:20210901T190413Z UID:9311-1638878400-1638882000@rna.umich.edu SUMMARY:Chase Beisel\, Helmholtz Center for infection Research DESCRIPTION:“Multiplexable RNA Detection by Cas9 Paves the Way for Novel COVID Testing Method”\nChase Beisel\, Helmholtz Center for infection Research\nDepartment of Biological Chemistry seminar\nLocation: 3330 MS I\nHosts: Yan Zhang and Nils Walter URL:https://rna.umich.edu/events/chase-beisel-helmholtz-center-for-infection-research/ END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20211213T160000 DTEND;TZID=America/Detroit:20211213T170000 DTSTAMP:20260403T160040 CREATED:20210827T134607Z LAST-MODIFIED:20211210T172017Z UID:9275-1639411200-1639414800@rna.umich.edu SUMMARY:RNA Faculty Candidate Seminar: Paul Donlin-Asp\, Max Planck Institute for Brain Research DESCRIPTION:“Resolving the localisation and dynamics of mRNA and protein synthesis within neurons”\nPaul Donlin-Asp\, Ph.D.\nPostdoctoral Researcher\nDepartment of Synaptic Plasticity\nMax Planck Institute for Brain Research \n  \n  \n  \n  \nFlyer in PDF \nCo-Host: The Department of Molecular\, Cellular\, and Developmental Biology \nHybrid Seminar:\nIn-person: Biomedical Science Research Building (BSRB)\, ABC Seminar rooms\nZoom: https://umich.zoom.us/webinar/register/WN_tZjYxppSQwaDVTYIuC0jkw \nKeywords: mRNA dynamics\, local protein synthesis\, neurons\, neuronal cell biology\, synaptic plasticity\, in vivo imaging \nIn-person event COVID guidelines URL:https://rna.umich.edu/events/donlin-asp/ CATEGORIES:Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20211215T160000 DTEND;TZID=America/Detroit:20211215T170000 DTSTAMP:20260403T160040 CREATED:20210901T192352Z LAST-MODIFIED:20210901T192352Z UID:9335-1639584000-1639587600@rna.umich.edu SUMMARY:RNA Collaborative Seminar Series // Host: Harvard Medical School Initiative for RNA Medicine DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-harvard-medical-school-initiative-for-rna-medicine/ END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20211220T160000 DTEND;TZID=America/Detroit:20211220T170000 DTSTAMP:20260403T160040 CREATED:20210827T134635Z LAST-MODIFIED:20211210T212954Z UID:9277-1640016000-1640019600@rna.umich.edu SUMMARY:RNA Faculty Candidate Seminar: Dr. Alisha 'Jonesy' Jones\, Institute of Structural Biology\, Helmholtz Zentrum Munich DESCRIPTION:“Modulation of the MALT1 pre-mRNA structure by hnRNP proteins regulates T cell activation”\nDr. Alisha ‘Jonesy’ Jones\nPostdoctoral Researcher\nInstitute of Structural Biology\nHelmholtz Zentrum Munich \n  \n  \nFlyer in PDF \nCo-Hosts: The Center for RNA Biomedicine\, Department of Biological Chemistry\, and the Program in Biophysics \nHybrid Seminar:\nIn-person: Biomedical Science Research Buliding (BSRB)\, ABC Seminar rooms\nZoom: https://umich.zoom.us/webinar/register/WN_3yrQ47UuTKKuzigbe38Sww \nKeywords: pre-mRNA\, hnRNP\, NMR\, SHAPE\, structure \nAbstract: Alternative splicing is controlled by differential binding of trans-acting RNA binding proteins (RBPs) to cis-regulatory pre-mRNA elements. How pre-mRNA secondary structure affects recognition by RBPs and determines alternative exon usage is poorly understood. The MALT1 paracaspase is a key component of signaling pathways that mediate innate and adaptive immune responses. Alternative splicing of MALT1 exon7 is critical for controlling optimal T cell activation. Here\, we demonstrate that MALT1 pre-mRNA splicing depends on RNA structural elements that shield the splice sites of the alternatively spliced exon7. The RBPs hnRNP U and hnRNP L bind comparably and competitively to identical stem-loop RNA structures flanking the 5’ and 3’ splice sites of MALT1 exon7. While hnRNP U stabilizes RNA stem-loop conformations that maintain exon7 skipping\, hnRNP L destabilizes these RNA elements to facilitate recruitment of the essential splicing factor U2AF2 to promote exon7 inclusion. This work represents a paradigm for the control of splice site selection by differential RBP binding and modulation of pre-mRNA structure. \nIn-person event COVID guidelines URL:https://rna.umich.edu/events/alisha-jonesy-jones/ CATEGORIES:Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20220110T160000 DTEND;TZID=America/Detroit:20220110T170000 DTSTAMP:20260403T160040 CREATED:20211130T161429Z LAST-MODIFIED:20220105T212127Z UID:9805-1641830400-1641834000@rna.umich.edu SUMMARY:RNA Faculty Candidate Seminar: Rachel Niederer\, Yale School of Medicine DESCRIPTION:“Uncovering novel translational control elements within 5′ UTRs”\nRachel Niederer\, Ph.D.\nPostdoctoral Researcher\nYale School of Medicine \n  \nCo-Hosts: The Center for RNA Biomedicine\, Department of Biological Chemistry\, and the Department of Human Genetics \nHybrid Seminar:\nIn-person: Biomedical Science Research Buliding (BSRB)\, ABC Seminar rooms\nZoom: https://umich.zoom.us/webinar/register/WN_qg4fyCUbQZyVu0oGrBLmWQ \nAbstract: Translational control of gene expression plays an essential role during development\, response to stress and a wide range of cellular processes. However\, the key mRNA features that distinguish efficiently translated from poorly translated mRNAs remain largely unknown. This talk will describe the development of direct analysis of ribosome targeting (DART) and its use both in discovering novel regulatory elements within 5′ untranslated regions (5′ UTRs) as well as revealing unexpected behaviors from features that were previously thought to be well understood. \nIn-person event COVID guidelines \nFlyer in PDF URL:https://rna.umich.edu/events/rachel-niederer/ CATEGORIES:Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20220131T160000 DTEND;TZID=America/Detroit:20220131T170000 DTSTAMP:20260403T160040 CREATED:20211130T164405Z LAST-MODIFIED:20220104T163046Z UID:9808-1643644800-1643648400@rna.umich.edu SUMMARY:RNA Faculty Candidate Seminar: Jailson (Jay) Brito Querido\, MRC Laboratory of Molecular Biology DESCRIPTION:“The scanning mechanism of mRNA translation initiation in humans”\nJailson (Jay) Brito Querido\, Ph.D.\nPostdoctoral Scientist\nMRC Laboratory of Molecular Biology\nCambridge\, UK \n  \n  \n  \nCo-Hosts: The Center for RNA Biomedicine\, Department of Biological Chemistry\, and the Program in Biophysics \nHybrid Seminar:\nIn-person: Biomedical Science Research Building (BSRB)\, ABC Seminar rooms\nZoom: https://umich.zoom.us/webinar/register/WN_pcS-fWIdSS-hDnKzoRhXeg \nKeywords: mRNA\, translation\, ribosome\, helicase\nAbstract: Decoding the genetic information into protein is fundamental for all kingdoms of life. It requires precise mechanisms to transcribe the DNA into mRNA\, which then can be translated by the ribosome to produce proteins. Translation initiation of eukaryotic mRNAs is a dynamic process regulated by over a dozen protein eukaryotic initiation factors (eIFs). This process starts with the binding of eukaryotic initiation factors eIF1\, eIF1A\, eIF3\, eIF5\, and a ternary complex of eIF2–GTP–tRNAiMet (TC) to the 40S small ribosomal subunit\, forming the 43S preinitiation complex (43S PIC). Once assembled\, the 43S PIC is recruited to the 5′ untranslated region (UTR) of mRNA by the multifactor cap-binding complex eIF4F\, forming the 48S initiation complex (48S). The 48S then scans along the 5′ UTR mRNA to locate a start codon. The eIF4F binding site in the 48S and how mRNA is inserted into the mRNA channel in the 40S small ribosomal subunit remained unknown. To gain insights into the molecular mechanism underlining the assembly of the 48S\, we used cryo-electron microscopy to determine the structure of a reconstituted human 48S. The structure sheds light on the early events of translation initiation complex assembly\, including how eIF4F interacts with the 43S during the scanning process. \nIn-person COVID Events Policy URL:https://rna.umich.edu/events/jay-brito-querido/ CATEGORIES:Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20220214T160000 DTEND;TZID=America/Detroit:20220214T170000 DTSTAMP:20260403T160040 CREATED:20211130T173006Z LAST-MODIFIED:20220204T174223Z UID:9812-1644854400-1644858000@rna.umich.edu SUMMARY:RNA Faculty Candidate Seminar: Margaret (Maggie) Rodgers\, Johns Hopkins University DESCRIPTION:“Mechanisms of co-transcriptional ribonucleoprotein assembly”\nMargaret (Maggie) Rodgers\, Ph.D.\nPostdoctoral Researcher\nJohns Hopkins University \n  \n  \n  \n  \nCo-Hosts: The Center for RNA Biomedicine\, Department of Biological Chemistry\, and the Program in Biophysics \nHybrid Seminar:\nIn-person: Biomedical Science Research Buliding (BSRB)\, ABC Seminar rooms\nZoom: https://umich.zoom.us/webinar/register/WN_Icqg8jRbQNGCBEFkj_u5Gw \nKeywords: RNA\, RNP\, ribosome assembly\, transcription\, single-molecule fluorescence \nSeminar flyer in PDF \nIn-person event COVID guidelines URL:https://rna.umich.edu/events/margaret-maggie-rodgers/ CATEGORIES:Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20220228T160000 DTEND;TZID=America/Detroit:20220228T170000 DTSTAMP:20260403T160040 CREATED:20220214T173408Z LAST-MODIFIED:20220214T212905Z UID:10113-1646064000-1646067600@rna.umich.edu SUMMARY:Seminar: John Prensner\, MD\, PhD DESCRIPTION:“Translating the cancer genome: dark matter proteins”\nJohn Prensner\, M.D.\, Ph.D.\nPhysician\, Dana-Farber/Boston Children’s Cancer and Blood Disorders Center\nInstructor of Pediatrics\, Harvard Medical School \n  \n  \nCo-Hosts: The Center for RNA Biomedicine\, Department of Computational Medicine & Bioinformatics\, and Department of Pediatric Oncology \nHybrid Seminar:\nIn-person: Biomedical Science Research Building (BSRB)\, ABC Seminar rooms\nZoom: https://umich.zoom.us/webinar/register/WN_bC6oy6VZTLy3ke4iMS2sKQ \nKeywords: cancer\, ribosome profiling\, functional genomics\, gene discovery \nAbstract: Although genomic analyses predict many non-canonical open reading frames (ORFs) in the human genome\, it is unclear whether they encode biologically active proteins in diseases such as cancer. Here\, we have developed functional genomics platforms to systematically interrogate non-canonical ORFs identified in ribosome profiling data. Using CRISPR loss-of-function screens in numerous human cancer models\, we define the frequency with which ORFs contribute to cell essentiality phenotypes. We further constructed large-scale ORF plasmid libraries to assess their capacity to encode a protein and induce cancer cell transcriptional changes. We pursued focused investigation of several uncharacterized protein-coding loci\, defining roles for the lncRNA-ORF GREP1 in breast cancer and the ASNSD1 upstream ORF (uORF) in medulloblastoma. Lastly\, we have assembled a pathway to bring such ORF candidates into the mainstream of biological research via an international consortium of gene database stakeholders. Together\, this work establishes non-canonical ORFs as critical mediators of cancer cell biology\, suggests their potential promise as therapeutic targets in cancer\, and outlines a path forward for dissemination of these ORFs amongst the global research community. \nIn-person COVID Events Policy URL:https://rna.umich.edu/events/john-prensner/ CATEGORIES:Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20220321T150000 DTEND;TZID=America/Detroit:20220321T160000 DTSTAMP:20260403T160040 CREATED:20220314T142057Z LAST-MODIFIED:20220314T183351Z UID:10183-1647874800-1647878400@rna.umich.edu SUMMARY:RNA Therapeutics Seminar\, Michelle Hastings\, Rosalind Franklin University DESCRIPTION:Inaugural RNA Therapeutics Seminar\nMichelle Hastings\, Ph.D.\nProfessor\, Cell Biology and Anatomy; Director\, Center for Genetic Diseases\nRosalind Franklin University of Medicine and Science \n  \n  \n  \nThis is an internal U-M event:\nIn-person: Palmer Commons\, Forum Hall\nZoom: https://umich.zoom.us/webinar/register/WN_mUj40sudTwmI6hXANnlJEg \nKeywords: pre-mRNA splicing\, Antisense oligonucleotides\, Usher syndrome\, Batten Disease\, lysosomal storage diseases \nSeminar flyer in PDF \nIn-person COVID Events Policy URL:https://rna.umich.edu/events/rna-therapeutics-seminar-michelle-hastings/ CATEGORIES:Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20220325T083000 DTEND;TZID=America/Detroit:20220325T160000 DTSTAMP:20260403T160040 CREATED:20210910T175731Z LAST-MODIFIED:20210910T175921Z UID:9401-1648197000-1648224000@rna.umich.edu SUMMARY:6th Annual RNA Symposium DESCRIPTION:Featured keynote speakers\nChris Burge\, MIT\nMichelle Hastings\, Rosalind Franklin University of Medicine and Science\nJohn Rinn\, University of Colorado-Boulder\nGisela Storz\, NIH\nJack Szostak\, University of Chicago \nFor more details\, visit: https://rna.umich.edu/2022-symposium/ \n  \n  \n\n\n \n \n Symposium Registration Form\n \n \n InstagramThis field is for validation purposes and should be left unchanged.Name*\n \n \n \n First\n \n \n \n \n Last\n \n \n TitleDepartmentEmail*\n \n PhoneWill you attend in-person or via Zoom?*\n \n In-person\n \n \n Zoom\n \n \n Both\n CAPTCHA\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n Δ URL:https://rna.umich.edu/events/6th-annual-rna-symposium/ CATEGORIES:Symposium END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20220328T160000 DTEND;TZID=America/Detroit:20220328T170000 DTSTAMP:20260403T160040 CREATED:20211206T182658Z LAST-MODIFIED:20220319T182647Z UID:9869-1648483200-1648486800@rna.umich.edu SUMMARY:RNA Innovation Seminar: Wendy Gilbert\, Ph.D. DESCRIPTION:“Marvelous U: Canonical and non-canonical functions of uridine modifying enzymes in health and disease”\nWendy Gilbert\, Ph.D.\nAssociate Professor of Molecular Biophysics and Biochemistry\nYale School of Medicine \nFlyer in PDF \n  \n  \n  \nHybrid Seminar\nIn-person: BSRB\, ABC Seminar Rooms\nZoom: https://umich.zoom.us/webinar/register/WN_P6PEipcsRPmG7cAL104K-Q \nKeywords:\nRNA modifications\, RNA processing\, RNA folding\, pre-mRNA\, tRNA\, translation\, cancer \nAbstract:\nRNA modifications are essential for human health—too much or too little leads to serious illnesses ranging from neurodevelopmental disorders to cancer. We are working to uncover the RNA targets of RNA modifying enzymes that are dysregulated in disease and to understand their molecular and organismal roles. Recent advances in detecting the modified nucleosides pseudouridine and dihydrouridine reveal complex landscapes that include pre-messenger RNA and diverse classes of noncoding RNA in yeast and human cells. I will give an update on our progress towards answering three questions: How are specific RNA sites selected for modification and how is this process regulated? What are the molecular consequences of mRNA and non-coding RNA modifications? How do specific defects in RNA metabolism result in organismal phenotypes\, including disease? URL:https://rna.umich.edu/events/wendy-gilbert/ END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20220411T160000 DTEND;TZID=America/Detroit:20220411T170000 DTSTAMP:20260403T160040 CREATED:20211206T183206Z LAST-MODIFIED:20230129T215823Z UID:9872-1649692800-1649696400@rna.umich.edu SUMMARY:RNA Innovation Seminar: Silvi Rouskin\, Ph.D. DESCRIPTION:“Alternative structures of the SARS-CoV-2 RNA genome control gene expression and offer therapeutic strategies”\nSilvi Rouskin\, Ph.D.\nAssistant Professor\nHarvard Medical School \n  \n  \n  \n  \nVirtual Seminar via Zoom: https://umich.zoom.us/webinar/register/WN_zi3UlKucR6G51hEA_33Exg \nKeywords:\nTBA \nAbstract:\nTBA URL:https://rna.umich.edu/events/silvi-rouskin-2022/ END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20220418T120000 DTEND;TZID=America/Detroit:20220418T130000 DTSTAMP:20260403T160040 CREATED:20211206T183726Z LAST-MODIFIED:20220412T180710Z UID:9876-1650283200-1650286800@rna.umich.edu SUMMARY:RNA Innovation Seminar: Paul Magwene\, Ph.D.\, Duke University DESCRIPTION:“Signaling Pathway Variation and Evolutionary Hotspots in the Fungi”\nPaul Magwene\, Ph.D. \nProfessor of Biology\nDirector\, Computational Biology & Bioinformatics (CBB)\nDuke University \n  \nPDF Flier \nHybrid Seminar\nIn-person: BSRB\, ABC Seminar Rooms\nZoom: https://umich.zoom.us/webinar/register/WN_7wk1SlRdQ_e04VnwcjVV7g \nKeywords:\npopulation genomics\, statistical genetics\, gene networks\, microbial pathogenesis \nAbstract:\nEvolutionarily conserved signal transduction pathways\, such as Ras-cAMP-PKA\, calcineurin\, and TOR signaling\, are primary regulators of stress responses and morphogenetic processes across the fungal tree of life. From an evolutionary perspective\, these pathways are expected to be under relatively strong stabilizing selection\, as loss-of-function mutations (LoF) in these pathways typically lead to reduced growth rates and increased sensitivity to environmental stresses. We have carried out comparative population genomic analyses of signaling pathway LoF alleles for multiple fungal species\, and find that several pathways exhibit unusually high frequencies of naturally occurring putative LoF alleles. We discuss the implications of this finding for the evolutionary lability of signaling pathways in the fungi\, and combine information on loss-of-function alleles with related evidence from QTL mapping and experimental evolution studies to identify pathways that may act as “evolutionary hotspots” for adaptation to novel environments. URL:https://rna.umich.edu/events/paul-magwene/ END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20220509T160000 DTEND;TZID=America/Detroit:20220509T170000 DTSTAMP:20260403T160040 CREATED:20211216T202325Z LAST-MODIFIED:20220502T191433Z UID:9963-1652112000-1652115600@rna.umich.edu SUMMARY:RNA Innovation Seminar: Sika Zheng\, UC Riverside\, School of Medicine DESCRIPTION:“Unexpected determinants of neuronal identity and properties: the curious cases of PTBP1\, PTBP2\, and neuronal splicing”\nSika Zheng\, Ph.D. \nAssociate Professor\, Director of Center for RNA Biology and Medicine\nUC Riverside\, School of Medicine \n  \nFlyer in PDF \n  \nVirtual Seminar\nZoom: https://umich.zoom.us/webinar/register/WN_dltbxWdHQ5KrC3rTl4hqLQ \nAbstract:\nAlternative splicing is the major contributor to transcriptome diversity\, but splicing is noisy and to what extend alternative splicing regulation is indispensable for biolgical processes has been controversial. Our studies have revealed the regulation and function of neural-specific splicing in shaping neuronal identity and estalishing neurons’ two unique attributes: 1. Axonogenesis (Only neurons but no other cell types have one and single axon); 2. Neuronal longevity (Neurons are the most long-lived cell types). We show that obtaining these neuronal features is coordinated by RNA binding proteins PTBP1 and PTBP2\, while PTBP1 was suggested by others to be a reprogramming factor of neuronal fate. I will discuss the regulatory mechanism of neural specific splicing underlying neurogensis and maturation. \nReferences:\nZhang M\, Ergin V\, Lin L\, Stork C\, Chen L\, Zheng S. Neuron. 2019 Feb 20;101(4):690-706.e10.\nErgin V\, Zheng S. J Mol Biol. 2020 Jun 26;432(14):4154-4166.\nZheng S. Wiley Interdiscip Rev RNA. 2020 Jul;11(4):e1585.\nLin L\, Zhang M\, Stoilov P\, Chen L\, Zheng S. Neuron. 2020 Sep 23;107(6):1180-1196.e8.\nVuong J\, Ergin V\, Zheng S. Nature Communications (accepted) URL:https://rna.umich.edu/events/sika-zheng/ CATEGORIES:Seminar END:VEVENT BEGIN:VEVENT DTSTART;TZID=America/Detroit:20220516T160000 DTEND;TZID=America/Detroit:20220516T170000 DTSTAMP:20260403T160040 CREATED:20220218T163352Z LAST-MODIFIED:20220506T161447Z UID:10130-1652716800-1652720400@rna.umich.edu SUMMARY:RNA Innovation Seminar: Daniel O’Reilly DESCRIPTION:“An Academic Approach to Oligonucleotide Therapeutics”\nDaniel O’Reilly Ph.D.\, MRSC\nPost-Doctoral Associate\nKhvorova Lab\nRNA Therapeutics Institute\nUniversity of Massachusetts Medical School \nFlyer in PDF \n  \nHYBRID SEMINAR:\nIn-person: BSRC\, ABC seminar rooms\nZoom: https://umich.zoom.us/webinar/register/WN_dM1a1aKVTM2KfwiieutOWg \nKeywords: Oligonucleotides\, Chemical Modifications\, RNA\, Huntington’s Disease \nAbstract: Nucleic acids (NA) are becoming the third major pillar of therapeutic modalities on par with small molecules and biologics. The diversity of NA molecular mechanisms\, ranging from vaccines\, antisense\, short interfering RNA (siRNAs)\, and guide RNA for CRISPR gene editing systems\, enable impact on most aspects of cellular biology and thus human medicine. The foundation behind the recent oligonucleotides’ clinical success is fundamental chemical innovations in RNA stability\, delivery\, and synthesis.\nOligonucleotides are informational drugs; thus\, if chemical architectures supporting safe and efficient delivery to the tissue of interest are achieved\, they can be easily reprogrammed to modulate any gene expression on demand\, creating an opportunity for academic institutions to drive therapeutic innovation. However\, the process is limited by access to oligonucleotide chemistry and synthetic expertise.\nIn the first half of the talk\, I will share the experience of building and running Nucleic Acid Chemistry Center in a context of a large academic institution. The NACC provides access to therapeutic quality screening leads and large manufacturing of preclinical compounds for the academic community. The impact of the NACC and chemical innovation will be discussed in the context of two significant projects. First\, I will discuss the systematic structure-activity relationship study of chemical modifications to modulate RISC loading and cleavage. Screening 1200 siRNA variants allow for defining the chemical and thermodynamic rules for RISC assembly. URL:https://rna.umich.edu/events/daniel-oreilly/ CATEGORIES:Seminar END:VEVENT END:VCALENDAR