BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Center for RNA Biomedicine - ECPv6.15.17//NONSGML v1.0//EN
CALSCALE:GREGORIAN
METHOD:PUBLISH
X-WR-CALNAME:Center for RNA Biomedicine
X-ORIGINAL-URL:https://rna.umich.edu
X-WR-CALDESC:Events for Center for RNA Biomedicine
REFRESH-INTERVAL;VALUE=DURATION:PT1H
X-Robots-Tag:noindex
X-PUBLISHED-TTL:PT1H
BEGIN:VTIMEZONE
TZID:America/Detroit
BEGIN:DAYLIGHT
TZOFFSETFROM:-0500
TZOFFSETTO:-0400
TZNAME:EDT
DTSTART:20200308T070000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:-0400
TZOFFSETTO:-0500
TZNAME:EST
DTSTART:20201101T060000
END:STANDARD
BEGIN:DAYLIGHT
TZOFFSETFROM:-0500
TZOFFSETTO:-0400
TZNAME:EDT
DTSTART:20210314T070000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:-0400
TZOFFSETTO:-0500
TZNAME:EST
DTSTART:20211107T060000
END:STANDARD
BEGIN:DAYLIGHT
TZOFFSETFROM:-0500
TZOFFSETTO:-0400
TZNAME:EDT
DTSTART:20220313T070000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:-0400
TZOFFSETTO:-0500
TZNAME:EST
DTSTART:20221106T060000
END:STANDARD
BEGIN:DAYLIGHT
TZOFFSETFROM:-0500
TZOFFSETTO:-0400
TZNAME:EDT
DTSTART:20230312T070000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:-0400
TZOFFSETTO:-0500
TZNAME:EST
DTSTART:20231105T060000
END:STANDARD
END:VTIMEZONE
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20210630T160000
DTEND;TZID=America/Detroit:20210630T170000
DTSTAMP:20260403T141744
CREATED:20210415T215318Z
LAST-MODIFIED:20210609T182833Z
UID:8538-1625068800-1625072400@rna.umich.edu
SUMMARY:RNA Collaborative Seminar Series // Host: University of Michigan
DESCRIPTION:REGISTRATION REQUIRED: https://umich.zoom.us/webinar/register/WN_6OEQ6sDAQ0-21GHm6d7VEQ\n\n“Dynamic multivalent interactions drive mammalian RNA regulation”\n \nSethu Pitchiaya\, Ph.D.\nDepartment of Urology \n\n  \n  \n  \n  \n  \n  \n  \n\n\n\n“Characterizing cellular RNA-protein interaction networks with chemical probes”\nChase Weidmann\, Ph.D.\nDepartment of Biological Chemistry \n  \n  \n  \n  \n  \n  \n  \n\nFor the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series
URL:https://rna.umich.edu/events/rna-collaborative-pitchiaya-weidmann/
CATEGORIES:Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20210714T090000
DTEND;TZID=America/Detroit:20210714T100000
DTSTAMP:20260403T141745
CREATED:20210415T215433Z
LAST-MODIFIED:20210415T215433Z
UID:8540-1626253200-1626256800@rna.umich.edu
SUMMARY:RNA Collaborative Seminar Series // Host: iRNA @ Istituto Italiano di Tecnologia (IIT)
DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series
URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-irna-istituto-italiano-di-tecnologia-iit/
CATEGORIES:Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20210728T090000
DTEND;TZID=America/Detroit:20210728T100000
DTSTAMP:20260403T141745
CREATED:20210415T215545Z
LAST-MODIFIED:20210426T152555Z
UID:8542-1627462800-1627466400@rna.umich.edu
SUMMARY:RNA Collaborative Seminar Series // Host: MD Anderson Center for RNA Interference and Non-Coding RNAs
DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series
URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-md-anderson-center-for-rna-interference-and-non-coding-rnas/
CATEGORIES:Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20210811T160000
DTEND;TZID=America/Detroit:20210811T170000
DTSTAMP:20260403T141745
CREATED:20210415T215644Z
LAST-MODIFIED:20210517T161927Z
UID:8544-1628697600-1628701200@rna.umich.edu
SUMMARY:RNA Collaborative Seminar Series // Host: RiboClub Sherbrooke
DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series
URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-tba-3/
CATEGORIES:Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20210825T090000
DTEND;TZID=America/Detroit:20210825T100000
DTSTAMP:20260403T141745
CREATED:20210415T215751Z
LAST-MODIFIED:20210426T152519Z
UID:8546-1629882000-1629885600@rna.umich.edu
SUMMARY:RNA Collaborative Seminar Series // Host: Institute of Molecular Biology (IMB)
DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series
URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host/
CATEGORIES:Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20210907T120000
DTEND;TZID=America/Detroit:20210907T130000
DTSTAMP:20260403T141745
CREATED:20210901T191230Z
LAST-MODIFIED:20210901T191230Z
UID:9318-1631016000-1631019600@rna.umich.edu
SUMMARY:Dipali Sashital\, Iowa State University
DESCRIPTION:“Achieving and Balancing Specificity in the CRISPR-Cas Systems”\nDipali Sashital\, Iowa State University\nDepartment of Biological Chemistry seminar\nZoom link: https://umich.zoom.us/j/91821866007\nHosts: Yan Zhang and Nils Walter
URL:https://rna.umich.edu/events/dipali-sashital-iowa-state-university/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20210908T160000
DTEND;TZID=America/Detroit:20210908T170000
DTSTAMP:20260403T141745
CREATED:20210901T191545Z
LAST-MODIFIED:20210901T191545Z
UID:9320-1631116800-1631120400@rna.umich.edu
SUMMARY:RNA Collaborative Seminar Series // Host: Rochester
DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series
URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-rochester/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20210910T160000
DTEND;TZID=America/Detroit:20210910T170000
DTSTAMP:20260403T141745
CREATED:20210901T192746Z
LAST-MODIFIED:20210901T192746Z
UID:9337-1631289600-1631293200@rna.umich.edu
SUMMARY:Bioinnitiatives in Brain Cancer (BIBC) | Castro & Lowenstien
DESCRIPTION:BIBC Hybrid Seminar Series: Dr. Pedro Lowenstein and Dr. Maria G. Castro\nDATE: Friday\, September 10\, 2021\nTIME: 4:00 pm—5:00 pm\nLOCATION: NCRC\, B10 Research Auditorium\nDr. Maria G. Castro and Dr. Pedro Lowenstein\, Richard C. Schneider Collegiate Professors of Neurosurgery will be presenting their novel work studying glioma invasion\, migration\, growth\, and the immune tumor microenvironment. \nThe BIBC seminars will showcase the multidisciplinary research of faculty studying brain cancer biology and novel technologies that can be used for brain cancer diagnosis and treatment. The seminars will be held on campus with a hybrid option. If you have not already received building access for NCRC\, enter through the Building 10 front entrance. \nAnyone attending the seminar in person must complete the ResponsiBLUE questionnaire and show the screening check when entering the research auditorium. At this time\, masks are required for all individuals regardless of vaccination status. We will update this event if University policy changes with regards to in-person events.
URL:https://rna.umich.edu/events/bioinnitiatives-in-brain-cancer-bibc-castro-lowenstien/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20210913T160000
DTEND;TZID=America/Detroit:20210913T170000
DTSTAMP:20260403T141745
CREATED:20210827T134151Z
LAST-MODIFIED:20210907T135538Z
UID:9263-1631548800-1631552400@rna.umich.edu
SUMMARY:RNA Innovation Seminar\, Rhiju Das\, Ph.D.
DESCRIPTION:“Recent improvements in modeling and design of RNA-only structures”\nRhiju Das\, Ph.D.\nAssociate Professor\nDepartments of Biochemistry and (by courtesy) Physics\nStanford University \nRegistration: https://umich.zoom.us/webinar/register/WN_i7vWSsxDQfSVbQP1C7HGSQ \n  \nAbstract:\nThe discovery and design of biologically important RNA molecules is outpacing three-structural characterization. I’ll describe results from my and Wah Chiu’s groups that demonstrate that cryo-electron microscopy can resolve maps of several kinds of RNA-only systems. These maps enable subnanometer-resolution 3D coordinate estimation when complemented with multidimensional chemical mapping and Rosetta DRRAFTER computational modeling. If time allows\, I’ll describe work from the Eterna project to stabilize mRNA molecules to help accelerate worldwide COVID immunization.
URL:https://rna.umich.edu/events/rhiju-das/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20210914T120000
DTEND;TZID=America/Detroit:20210914T130000
DTSTAMP:20260403T141745
CREATED:20210901T191030Z
LAST-MODIFIED:20210907T132755Z
UID:9316-1631620800-1631624400@rna.umich.edu
SUMMARY:BiolChem Seminar: Jason McLellan\, University of Texas at Austin
DESCRIPTION:“Structure-Function Studies of the SARS-CoV-2 Spike and Development of COVID-19 Interventions”\nJason McLellan\, University of Texas at Austin\nDepartment of Biological Chemistry seminar\nLocation: 3330 MS I\nHost: Janet Smith
URL:https://rna.umich.edu/events/jason-mclellan-university-of-texas-at-austin/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20210921T120000
DTEND;TZID=America/Detroit:20210921T130000
DTSTAMP:20260403T141745
CREATED:20210901T190819Z
LAST-MODIFIED:20210908T165943Z
UID:9313-1632225600-1632229200@rna.umich.edu
SUMMARY:BiolChem Seminar: Alexis Komor\, UCSD
DESCRIPTION:“Base Editing-Performing Chemistry on the Genome”\nAlexis Komor\, UCSD\nDepartment of Biological Chemistry seminar\nLocation: Zoom Webinar https://umich.zoom.us/j/91821866007\nHosts: Yan Zhang and Nils Walter
URL:https://rna.umich.edu/events/alexis-komor/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20210922T160000
DTEND;TZID=America/Detroit:20210922T170000
DTSTAMP:20260403T141745
CREATED:20210901T191748Z
LAST-MODIFIED:20210901T191748Z
UID:9322-1632326400-1632330000@rna.umich.edu
SUMMARY:RNA Collaborative Seminar Series // Host: NCI RNA Biology Initiative
DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series
URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-nci-rna-biology-initiative/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20210924T100000
DTEND;TZID=America/Detroit:20210924T110000
DTSTAMP:20260403T141745
CREATED:20210901T193132Z
LAST-MODIFIED:20210901T193132Z
UID:9344-1632477600-1632481200@rna.umich.edu
SUMMARY:Jayakrishnan Nandakumar\, MCDB
DESCRIPTION:“Structure of a meiosis-specific complex central to BRCA2 localization at recombination sites”\nJayakrishnan Nandakumar\, Ph.D.\nU-M LSI Structure Series\nLocation: LSI Library
URL:https://rna.umich.edu/events/jk-nandakumar/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20211004T160000
DTEND;TZID=America/Detroit:20211004T170000
DTSTAMP:20260403T141745
CREATED:20210827T134403Z
LAST-MODIFIED:20210930T134032Z
UID:9267-1633363200-1633366800@rna.umich.edu
SUMMARY:RNA Innovation Seminar: Byron Purse\, Ph.D.
DESCRIPTION:“Fluorescent nucleoside analogues with new properties”\nByron Purse\, Ph.D.\nAssociate Professor\nOrganic Chemistry\nSan Diego State University \n  \n  \n  \n  \nHYBRID SEMINAR:\nIn-person: Forum Hall\, Palmer Commons\nZoom: https://umich.zoom.us/webinar/register/WN__vvE2dtHQi-R3h05JUHBzQ \nFLYER IN PDF \nAbstract:\nFluorescent nucleoside analogues (FNAs) are powerful probes for studying the structure and dynamics of nucleic acids\, which are vital to understanding RNA function\, DNA damage repair\, nucleic acid–protein interactions\, regulatory mechanisms for gene expression\, and other aspects of nucleic acid function. Existing FNAs are prone to quenching by base pairing and stacking\, are clustered at the blue–green end of the visible spectrum\, and have limited brightness as compared with conventional fluorophores. Studies of nucleic acid function would benefit greatly from overcoming these limitations. We have designed\, synthesized\, and studied a series of fluorescent pyrimidine analogues\, aiming to address these limitations and develop a detailed understanding of the relationships between chemical structure and fluorescent responses to local environment in nucleic acids. Included in this series is a tricyclic cytidine analogue DEAtC that is nearly non-fluorescent as a nucleoside\, but responds to matched base pairing and stacking with a fluorescence turn-on. A chlorinated tricyclic cytidine 8-Cl-tCO reports on local environment by changes in the vibrational fine structure of its emission spectra. To address the problem of limited brightness\, we have design and synthesized a new NFA that we call ABN\, which has a conjugated push–pull system similar to those found in bright fluorophores such as rhodamines. ABN is the brightest known FNA when present in duplex nucleic acids\, and it is readily detected in single-molecule fluorescence measurements using both 1-photon and 2-photon excitation. Collectively\, these FNAs offer new capabilities for biophysical studies on nucleic acids. Comparisons of their structure and properties help to reveal mechanisms for fluorescence changes in response to local environment in nucleic acids.
URL:https://rna.umich.edu/events/byron-purse/
CATEGORIES:Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20211005T150000
DTEND;TZID=America/Detroit:20211005T160000
DTSTAMP:20260403T141745
CREATED:20210901T193912Z
LAST-MODIFIED:20210929T155531Z
UID:9346-1633446000-1633449600@rna.umich.edu
SUMMARY:Human Genetics Seminar: Rajesh Rao\, Ophthalmology & Visual Science\, U-M Medical School
DESCRIPTION:“Genetics through the Lens of an Ophthalmologist and Vision Scientist”\nRajesh C. Rao\, M.D.\nLeonard G. Miller Professor\nAssociate Professor\nDepartment of Ophthalmology & Visual Sciences\nDepartment of Pathology \n  \nDepartment of Human Genetics seminar\nZOOM MEETING ID: 947 5800 7294\nPASSCODE: 900076\nhttps://umich.zoom.us/j/94758007294?pwd=Sk9VMGlnTXRZWWJwbnZCZUx4MlI0QT09 \nFLYER IN PDF
URL:https://rna.umich.edu/events/rajesh-rao/
CATEGORIES:Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20211006T090000
DTEND;TZID=America/Detroit:20211006T100000
DTSTAMP:20260403T141745
CREATED:20210901T191902Z
LAST-MODIFIED:20210901T191902Z
UID:9325-1633510800-1633514400@rna.umich.edu
SUMMARY:RNA Collaborative Seminar Series // Host: NUS-CSI Singapore RNA Biology Center
DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series
URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-nus-csi-singapore-rna-biology-center/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20211012T120000
DTEND;TZID=America/Detroit:20211012T130000
DTSTAMP:20260403T141745
CREATED:20210901T185022Z
LAST-MODIFIED:20210908T170009Z
UID:9294-1634040000-1634043600@rna.umich.edu
SUMMARY:BiolChem Seminar: Sam Sternberg\, Columbia University
DESCRIPTION:“Functional Interplay Between CRISPR-Cas and Transposable Elements”\nSam Sternberg\, Columbia University\nDepartment of Biological Chemistry seminar\nLocation: 3330 MS I\nHosts: Yan Zhang and Nils Walter
URL:https://rna.umich.edu/events/sam-sternberg/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20211018T160000
DTEND;TZID=America/Detroit:20211018T170000
DTSTAMP:20260403T141745
CREATED:20210827T134441Z
LAST-MODIFIED:20210930T165005Z
UID:9269-1634572800-1634576400@rna.umich.edu
SUMMARY:RNA Innovation Seminar: Tim Stasevich\, Ph.D.
DESCRIPTION:“Imaging single-mRNA translation dynamics in living color”\nTim Stasevich\, Ph.D.\nAssociate Professor\nDean and Pingping Tsao Professor of Biochemistry\nCSU Monfort Professor\nBoettcher Investigator\nColorado State University \nRegistration: https://umich.zoom.us/webinar/register/WN_OzTuwf2zRN-6zCZk1pA_lQ \n  \nFlyer in PDF \nKeywords:\ntranslational regulation\, gene expression\, fluorescence microscopy\, intrabodies\, single-molecule imaging \nAbstract:\nMy lab is creating technology to image mRNA translation in real time and with single-molecule precision in living cells. In this talk\, I will introduce our technology and describe how it can be used to amplify fluorescence from newly synthesized proteins as they are being translated from single mRNAs. I will show how we quantify these signals to determine the size\, shape\, subcellular localization\, and mobilities of mRNA translation sites\, as well as their protein synthesis dynamics. I will then highlight a few recent applications of our technology\, focusing mainly on a new biosensor we have developed to quantify how individual regulatory factors impact single mRNA translation dynamics. Using this biosensor\, we provide evidence that human Argonaute2 (Ago2) shuts down translation by down regulating translation initiation on the minutes timescale and helping usher translationally silent mRNAs into P-bodies on the hours timescale. I will conclude by discussing new fluorescent intrabodies my lab is engineering to light up nascent and mature proteins in multiple colors. As these intrabodies can be encoded on plasmids\, they can easily be adapted by other labs to image gene activity in diverse living systems. \nTimothy J. Stasevich is an Associate Professor in the Department of Biochemistry and Molecular Biology at Colorado State University (CSU). His lab uses a combination of advanced fluorescence microscopy\, genetic engineering\, and computational modeling to study the dynamics of gene regulation in living mammalian cells. His lab helped pioneer the imaging of real-time single-mRNA translation dynamics in living cells1. Dr. Stasevich received his B.S. in Physics and Mathematics from the University of Michigan\, Dearborn\, and his Ph. D. in Physics from the University of Maryland\, College Park. He transitioned into experimental biophysics as a post-doctoral research fellow in the laboratory of Dr. James G. McNally at the National Cancer Institute. During this time\, he developed technology based on fluorescence microscopy to help establish gold-standard measurements of live-cell protein dynamics. Dr. Stasevich next moved to Osaka University\, where he worked with Dr. Hiroshi Kimura as a Japan Society for the Promotion of Science Foreign Postdoctoral Research Fellow. While there\, he helped create technology to image endogenous proteins and their post-translation modifications in vivo. This allowed him to image the live-cell dynamics of epigenetic histone modifications during gene activation for the first time2. Before joining the faculty at CSU\, Dr. Stasevich spent a year as a Visiting Fellow at the HHMI Janelia Research Campus\, where he applied superresolution fluorescence microscopy to improve the spatiotemporal resolution of endogenous protein imaging in live cells. \n1. Morisaki\, T. et al. Real-time quantification of single RNA translation dynamics in living cells. Science 352\, 1425–1429 (2016).\n2. Stasevich\, T. J. et al. Regulation of RNA polymerase II activation by histone acetylation in single living cells. Nature 516\, 272–275 (2014).
URL:https://rna.umich.edu/events/tim-stasevich/
CATEGORIES:Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20211020T090000
DTEND;TZID=America/Detroit:20211020T100000
DTSTAMP:20260403T141745
CREATED:20210901T192024Z
LAST-MODIFIED:20210901T192024Z
UID:9327-1634720400-1634724000@rna.umich.edu
SUMMARY:RNA Collaborative Seminar Series // Host: Helmholtz Institute for RNA-based Infection Research
DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series
URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-helmholtz-institute-for-rna-based-infection-research/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20211026T120000
DTEND;TZID=America/Detroit:20211026T130000
DTSTAMP:20260403T141745
CREATED:20210901T185327Z
LAST-MODIFIED:20210908T170049Z
UID:9297-1635249600-1635253200@rna.umich.edu
SUMMARY:BiolChem Seminar: Michael Terns\, University of Georgia
DESCRIPTION:“Know Thy Enemy: Making CRISPR Memories”\nMichael Terns\, University of Georgia\nDepartment of Biological Chemistry seminar\nLocation: 3330 MS I\nHosts: Yan Zhang and Nils Walter
URL:https://rna.umich.edu/events/michael-terns/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20211103T160000
DTEND;TZID=America/Detroit:20211103T170000
DTSTAMP:20260403T141745
CREATED:20210901T192135Z
LAST-MODIFIED:20210901T192135Z
UID:9329-1635955200-1635958800@rna.umich.edu
SUMMARY:RNA Collaborative Seminar Series // Host: UCSC Center for Molecular Biology of RNA
DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series
URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-ucsc-center-for-molecular-biology-of-rna/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20211108T160000
DTEND;TZID=America/Detroit:20211108T170000
DTSTAMP:20260403T141745
CREATED:20210827T134511Z
LAST-MODIFIED:20211025T164624Z
UID:9271-1636387200-1636390800@rna.umich.edu
SUMMARY:RNA Innovation Seminar: Zhonggang Hou & Jun Hee Lee
DESCRIPTION:“Harnessing diverse compact CRISPR-Cas3 for long-range genome engineering”\nZhonggang Hou\, Ph.D.\nResearch Investigator\nBiological Chemistry \nABSTRACT: Leading CRISPR-Cas technologies employ Cas9 and Cas12 enzymes that generate RNA-guided dsDNA breaks. Yet\, the most abundant microbial adaptive immune systems\, Type I CRISPR\, is under-exploited for eukaryotic application. I will discuss our effort on adopting the first minimal CRISPR-Cas3 from Neisseria Type I-C system\, to create targeted large chromosomal deletions in human cells. RNP delivery of the processive Cas3 nuclease and target recognition complex Cascade\, gave up to 90% editing efficiency. Unexpectedly\, Type I-C Cascade assembly in bacteria requires a previous unknown internal translation product Cas11 from within the cas8 gene. Our data show that expression of a separately encoded Cas11 is the key to enable plasmid- and mRNA- based editing in human cells. We demonstrate that “supplying cas11” is a universal strategy to harness divergent and streamlined Type I-C\, I-D and I-B editors with distinct PAM preferences and guide orthogonality. Our findings expand the CRISPR toolbox for long-range genome engineering. \nKEYWORDS: CRISPR; genome editing; Cascade; Cas3; Cas11; DNA targeting; crRNA; large deletion; Neisseria; genome engineering \n  \n  \n“Microscopic Examination of Spatial Transcriptome through Seq-Scope”\nJun Hee Lee\, Ph.D.\nAssociate Professor\nMolecular & Integrative Physiology \nKEYWORDS: Spatial Transcriptomics\, Seq-Scope \n  \nRegistration: https://umich.zoom.us/webinar/register/WN_qBK6mw7vQa6jOkZuS81_VQ \nFLYER IN PDF
URL:https://rna.umich.edu/events/zhonggang-hou-and-jun-hee-lee/
CATEGORIES:Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20211109T120000
DTEND;TZID=America/Detroit:20211109T130000
DTSTAMP:20260403T141745
CREATED:20210901T185606Z
LAST-MODIFIED:20210908T170232Z
UID:9302-1636459200-1636462800@rna.umich.edu
SUMMARY:BiolChem Seminar: Ailong Ke\, Cornell University
DESCRIPTION:“Structural Basis of Bacterial CRISPR Immunity”\nAilong Ke\, Cornell University\nDepartment of Biological Chemistry seminar\nLocation: 3330 MS I\nHosts: Yan Zhang and Nils Walter
URL:https://rna.umich.edu/events/ailong-ke/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20211117T160000
DTEND;TZID=America/Detroit:20211117T170000
DTSTAMP:20260403T141745
CREATED:20210901T192222Z
LAST-MODIFIED:20210901T192222Z
UID:9331-1637164800-1637168400@rna.umich.edu
SUMMARY:RNA Collaborative Seminar Series // Host: The RNA Institute\, University At Albany
DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series
URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-the-rna-institute-university-at-albany/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20211201T160000
DTEND;TZID=America/Detroit:20211201T170000
DTSTAMP:20260403T141745
CREATED:20210901T192308Z
LAST-MODIFIED:20210901T192308Z
UID:9333-1638374400-1638378000@rna.umich.edu
SUMMARY:RNA Collaborative Seminar Series // Host: Penn RNA Group - University of Pennsylvania
DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series
URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-penn-rna-group-university-of-pennsylvania/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20211207T120000
DTEND;TZID=America/Detroit:20211207T130000
DTSTAMP:20260403T141745
CREATED:20210901T190413Z
LAST-MODIFIED:20210901T190413Z
UID:9311-1638878400-1638882000@rna.umich.edu
SUMMARY:Chase Beisel\, Helmholtz Center for infection Research
DESCRIPTION:“Multiplexable RNA Detection by Cas9 Paves the Way for Novel COVID Testing Method”\nChase Beisel\, Helmholtz Center for infection Research\nDepartment of Biological Chemistry seminar\nLocation: 3330 MS I\nHosts: Yan Zhang and Nils Walter
URL:https://rna.umich.edu/events/chase-beisel-helmholtz-center-for-infection-research/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20211213T160000
DTEND;TZID=America/Detroit:20211213T170000
DTSTAMP:20260403T141745
CREATED:20210827T134607Z
LAST-MODIFIED:20211210T172017Z
UID:9275-1639411200-1639414800@rna.umich.edu
SUMMARY:RNA Faculty Candidate Seminar: Paul Donlin-Asp\, Max Planck Institute for Brain Research
DESCRIPTION:“Resolving the localisation and dynamics of mRNA and protein synthesis within neurons”\nPaul Donlin-Asp\, Ph.D.\nPostdoctoral Researcher\nDepartment of Synaptic Plasticity\nMax Planck Institute for Brain Research \n  \n  \n  \n  \nFlyer in PDF \nCo-Host: The Department of Molecular\, Cellular\, and Developmental Biology \nHybrid Seminar:\nIn-person: Biomedical Science Research Building (BSRB)\, ABC Seminar rooms\nZoom: https://umich.zoom.us/webinar/register/WN_tZjYxppSQwaDVTYIuC0jkw \nKeywords: mRNA dynamics\, local protein synthesis\, neurons\, neuronal cell biology\, synaptic plasticity\, in vivo imaging \nIn-person event COVID guidelines
URL:https://rna.umich.edu/events/donlin-asp/
CATEGORIES:Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20211215T160000
DTEND;TZID=America/Detroit:20211215T170000
DTSTAMP:20260403T141745
CREATED:20210901T192352Z
LAST-MODIFIED:20210901T192352Z
UID:9335-1639584000-1639587600@rna.umich.edu
SUMMARY:RNA Collaborative Seminar Series // Host: Harvard Medical School Initiative for RNA Medicine
DESCRIPTION:For the seminar details\, visit: https://www.rnasociety.org/rna-collaborative-seminar-series
URL:https://rna.umich.edu/events/rna-collaborative-seminar-series-host-harvard-medical-school-initiative-for-rna-medicine/
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20211220T160000
DTEND;TZID=America/Detroit:20211220T170000
DTSTAMP:20260403T141745
CREATED:20210827T134635Z
LAST-MODIFIED:20211210T212954Z
UID:9277-1640016000-1640019600@rna.umich.edu
SUMMARY:RNA Faculty Candidate Seminar: Dr. Alisha 'Jonesy' Jones\, Institute of Structural Biology\, Helmholtz Zentrum Munich
DESCRIPTION:“Modulation of the MALT1 pre-mRNA structure by hnRNP proteins regulates T cell activation”\nDr. Alisha ‘Jonesy’ Jones\nPostdoctoral Researcher\nInstitute of Structural Biology\nHelmholtz Zentrum Munich \n  \n  \nFlyer in PDF  \nCo-Hosts: The Center for RNA Biomedicine\, Department of Biological Chemistry\, and the Program in Biophysics \nHybrid Seminar:\nIn-person: Biomedical Science Research Buliding (BSRB)\, ABC Seminar rooms\nZoom: https://umich.zoom.us/webinar/register/WN_3yrQ47UuTKKuzigbe38Sww \nKeywords: pre-mRNA\, hnRNP\, NMR\, SHAPE\, structure \nAbstract: Alternative splicing is controlled by differential binding of trans-acting RNA binding proteins (RBPs) to cis-regulatory pre-mRNA elements. How pre-mRNA secondary structure affects recognition by RBPs and determines alternative exon usage is poorly understood. The MALT1 paracaspase is a key component of signaling pathways that mediate innate and adaptive immune responses. Alternative splicing of MALT1 exon7 is critical for controlling optimal T cell activation. Here\, we demonstrate that MALT1 pre-mRNA splicing depends on RNA structural elements that shield the splice sites of the alternatively spliced exon7. The RBPs hnRNP U and hnRNP L bind comparably and competitively to identical stem-loop RNA structures flanking the 5’ and 3’ splice sites of MALT1 exon7. While hnRNP U stabilizes RNA stem-loop conformations that maintain exon7 skipping\, hnRNP L destabilizes these RNA elements to facilitate recruitment of the essential splicing factor U2AF2 to promote exon7 inclusion. This work represents a paradigm for the control of splice site selection by differential RBP binding and modulation of pre-mRNA structure. \nIn-person event COVID guidelines
URL:https://rna.umich.edu/events/alisha-jonesy-jones/
CATEGORIES:Seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=America/Detroit:20220110T160000
DTEND;TZID=America/Detroit:20220110T170000
DTSTAMP:20260403T141745
CREATED:20211130T161429Z
LAST-MODIFIED:20220105T212127Z
UID:9805-1641830400-1641834000@rna.umich.edu
SUMMARY:RNA Faculty Candidate Seminar: Rachel Niederer\, Yale School of Medicine
DESCRIPTION:“Uncovering novel translational control elements within 5′ UTRs”\nRachel Niederer\, Ph.D.\nPostdoctoral Researcher\nYale School of Medicine \n  \nCo-Hosts: The Center for RNA Biomedicine\, Department of Biological Chemistry\, and the Department of Human Genetics \nHybrid Seminar:\nIn-person: Biomedical Science Research Buliding (BSRB)\, ABC Seminar rooms\nZoom: https://umich.zoom.us/webinar/register/WN_qg4fyCUbQZyVu0oGrBLmWQ \nAbstract: Translational control of gene expression plays an essential role during development\, response to stress and a wide range of cellular processes. However\, the key mRNA features that distinguish efficiently translated from poorly translated mRNAs remain largely unknown. This talk will describe the development of direct analysis of ribosome targeting (DART) and its use both in discovering novel regulatory elements within 5′ untranslated regions (5′ UTRs) as well as revealing unexpected behaviors from features that were previously thought to be well understood. \nIn-person event COVID guidelines \nFlyer in PDF
URL:https://rna.umich.edu/events/rachel-niederer/
CATEGORIES:Seminar
END:VEVENT
END:VCALENDAR