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X-WR-CALDESC:Events for Center for RNA Biomedicine
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DTSTART;TZID=America/Detroit:20211004T160000
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DTSTAMP:20260502T001839
CREATED:20210827T134403Z
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SUMMARY:RNA Innovation Seminar: Byron Purse\, Ph.D.
DESCRIPTION:“Fluorescent nucleoside analogues with new properties”\nByron Purse\, Ph.D.\nAssociate Professor\nOrganic Chemistry\nSan Diego State University \n  \n  \n  \n  \nHYBRID SEMINAR:\nIn-person: Forum Hall\, Palmer Commons\nZoom: https://umich.zoom.us/webinar/register/WN__vvE2dtHQi-R3h05JUHBzQ \nFLYER IN PDF \nAbstract:\nFluorescent nucleoside analogues (FNAs) are powerful probes for studying the structure and dynamics of nucleic acids\, which are vital to understanding RNA function\, DNA damage repair\, nucleic acid–protein interactions\, regulatory mechanisms for gene expression\, and other aspects of nucleic acid function. Existing FNAs are prone to quenching by base pairing and stacking\, are clustered at the blue–green end of the visible spectrum\, and have limited brightness as compared with conventional fluorophores. Studies of nucleic acid function would benefit greatly from overcoming these limitations. We have designed\, synthesized\, and studied a series of fluorescent pyrimidine analogues\, aiming to address these limitations and develop a detailed understanding of the relationships between chemical structure and fluorescent responses to local environment in nucleic acids. Included in this series is a tricyclic cytidine analogue DEAtC that is nearly non-fluorescent as a nucleoside\, but responds to matched base pairing and stacking with a fluorescence turn-on. A chlorinated tricyclic cytidine 8-Cl-tCO reports on local environment by changes in the vibrational fine structure of its emission spectra. To address the problem of limited brightness\, we have design and synthesized a new NFA that we call ABN\, which has a conjugated push–pull system similar to those found in bright fluorophores such as rhodamines. ABN is the brightest known FNA when present in duplex nucleic acids\, and it is readily detected in single-molecule fluorescence measurements using both 1-photon and 2-photon excitation. Collectively\, these FNAs offer new capabilities for biophysical studies on nucleic acids. Comparisons of their structure and properties help to reveal mechanisms for fluorescence changes in response to local environment in nucleic acids.
URL:https://rna.umich.edu/events/byron-purse/
CATEGORIES:Seminar
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DTSTART;TZID=America/Detroit:20211005T150000
DTEND;TZID=America/Detroit:20211005T160000
DTSTAMP:20260502T001839
CREATED:20210901T193912Z
LAST-MODIFIED:20210929T155531Z
UID:9346-1633446000-1633449600@rna.umich.edu
SUMMARY:Human Genetics Seminar: Rajesh Rao\, Ophthalmology & Visual Science\, U-M Medical School
DESCRIPTION:“Genetics through the Lens of an Ophthalmologist and Vision Scientist”\nRajesh C. Rao\, M.D.\nLeonard G. Miller Professor\nAssociate Professor\nDepartment of Ophthalmology & Visual Sciences\nDepartment of Pathology \n  \nDepartment of Human Genetics seminar\nZOOM MEETING ID: 947 5800 7294\nPASSCODE: 900076\nhttps://umich.zoom.us/j/94758007294?pwd=Sk9VMGlnTXRZWWJwbnZCZUx4MlI0QT09 \nFLYER IN PDF
URL:https://rna.umich.edu/events/rajesh-rao/
CATEGORIES:Seminar
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DTSTART;TZID=America/Detroit:20211018T160000
DTEND;TZID=America/Detroit:20211018T170000
DTSTAMP:20260502T001839
CREATED:20210827T134441Z
LAST-MODIFIED:20210930T165005Z
UID:9269-1634572800-1634576400@rna.umich.edu
SUMMARY:RNA Innovation Seminar: Tim Stasevich\, Ph.D.
DESCRIPTION:“Imaging single-mRNA translation dynamics in living color”\nTim Stasevich\, Ph.D.\nAssociate Professor\nDean and Pingping Tsao Professor of Biochemistry\nCSU Monfort Professor\nBoettcher Investigator\nColorado State University \nRegistration: https://umich.zoom.us/webinar/register/WN_OzTuwf2zRN-6zCZk1pA_lQ \n  \nFlyer in PDF \nKeywords:\ntranslational regulation\, gene expression\, fluorescence microscopy\, intrabodies\, single-molecule imaging \nAbstract:\nMy lab is creating technology to image mRNA translation in real time and with single-molecule precision in living cells. In this talk\, I will introduce our technology and describe how it can be used to amplify fluorescence from newly synthesized proteins as they are being translated from single mRNAs. I will show how we quantify these signals to determine the size\, shape\, subcellular localization\, and mobilities of mRNA translation sites\, as well as their protein synthesis dynamics. I will then highlight a few recent applications of our technology\, focusing mainly on a new biosensor we have developed to quantify how individual regulatory factors impact single mRNA translation dynamics. Using this biosensor\, we provide evidence that human Argonaute2 (Ago2) shuts down translation by down regulating translation initiation on the minutes timescale and helping usher translationally silent mRNAs into P-bodies on the hours timescale. I will conclude by discussing new fluorescent intrabodies my lab is engineering to light up nascent and mature proteins in multiple colors. As these intrabodies can be encoded on plasmids\, they can easily be adapted by other labs to image gene activity in diverse living systems. \nTimothy J. Stasevich is an Associate Professor in the Department of Biochemistry and Molecular Biology at Colorado State University (CSU). His lab uses a combination of advanced fluorescence microscopy\, genetic engineering\, and computational modeling to study the dynamics of gene regulation in living mammalian cells. His lab helped pioneer the imaging of real-time single-mRNA translation dynamics in living cells1. Dr. Stasevich received his B.S. in Physics and Mathematics from the University of Michigan\, Dearborn\, and his Ph. D. in Physics from the University of Maryland\, College Park. He transitioned into experimental biophysics as a post-doctoral research fellow in the laboratory of Dr. James G. McNally at the National Cancer Institute. During this time\, he developed technology based on fluorescence microscopy to help establish gold-standard measurements of live-cell protein dynamics. Dr. Stasevich next moved to Osaka University\, where he worked with Dr. Hiroshi Kimura as a Japan Society for the Promotion of Science Foreign Postdoctoral Research Fellow. While there\, he helped create technology to image endogenous proteins and their post-translation modifications in vivo. This allowed him to image the live-cell dynamics of epigenetic histone modifications during gene activation for the first time2. Before joining the faculty at CSU\, Dr. Stasevich spent a year as a Visiting Fellow at the HHMI Janelia Research Campus\, where he applied superresolution fluorescence microscopy to improve the spatiotemporal resolution of endogenous protein imaging in live cells. \n1. Morisaki\, T. et al. Real-time quantification of single RNA translation dynamics in living cells. Science 352\, 1425–1429 (2016).\n2. Stasevich\, T. J. et al. Regulation of RNA polymerase II activation by histone acetylation in single living cells. Nature 516\, 272–275 (2014).
URL:https://rna.umich.edu/events/tim-stasevich/
CATEGORIES:Seminar
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