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DTSTART;TZID=America/Detroit:20210518T160000
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SUMMARY:Seminar: Nick Ingolia\, UC Berkeley
DESCRIPTION:ZOOM LINK \n“Dissecting gene regulatory networks with CiBER-Seq”\nNicholas Ingolia\, PhD\nAssistant Professor\nDepartment of Molecular & Cell Biology\nUniversity of California\, Berkeley\nFaculty host:\nKristin Koutmou\, Ph.D.\nSeyhan N. Ege Assistant Professor of Chemistry\nDepartment of Chemistry\nDepartment of Chemistry Seminar \nABSTRACT: Cells integrate environmental signals and internal physiology to dynamically control gene expression. We have developed a technique to dissect this regulatory logic by linking targeted\, genome-wide genetic perturbations with a deep-sequencing readout that quantitatively measures the expression phenotype induced by each perturbation. Our approach combines CRISPR interference (CRISPRi) with barcoded expression reporters (CiBER-seq) to profile transcriptional\, translational\, and posttranslational regulatory responses\, connecting each guide in a genome-scale library with its individual phenotypic consequences. Applying CiBER-Seq to the well-studied integrated stress response (ISR) pathway recapitulated the known biology of this pathway and uncovered further triggers for ISR activation that were not previously appreciated. The quantitative phenotypic measurements from CiBER-Seq are well suited to epistasis analysis\, and we measure dual-perturbation phenotypes and compare patterns of genetic interaction to gain further insight into regulatory pathways. CiBER-Seq is an incisive tool for dissecting genetic networks\, and can be applied to study a wide range of biological processes\, using gene expression regulation as a sensitive and specific readout of the state of the cell. \n 
URL:https://rna.umich.edu/events/nick-ingolia-uc-berkeley/
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